Table 1.
Selectivity assessment of the four SYBR®Green qPCR assays: “iap-50-deg”, “prs-2-deg”, “hlyA-177” and “hlyA-146-deg-tronc”
| Genus | Species | Serogroup | GRAM | Origin | Reference | iap-50-deg | prs-2-deg | hlyA-177 | hlyA-146-deg-tronc |
|---|---|---|---|---|---|---|---|---|---|
| Listeria | monocytogenes | 1/2a | + | List-NRC | 10/13 | + | + | + | + |
| Listeria | monocytogenes | 1/2a | + | List-NRC | 10/14 | + | + | + | + |
| Listeria | monocytogenes | 1/2a | + | List-NRC | 10/15 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2a | + | List-NRC | 10/20 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2a | + | List-NRC | 10/21 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2a | + | List-NRC | 10/22 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2a | + | List-NRC | 10/23 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2a | + | List-NRC | ATCC 51772 | + | + | + | + |
| Listeria | monocytogenes | 1/2b | + | List-NRC | ATCC 51777 | + | + | + | + |
| Listeria | monocytogenes | 1/2b | + | List-NRC | 10/2 | + | + | + | + |
| Listeria | monocytogenes | 1/2b | + | List-NRC | 10/28 | + | + | + | + |
| Listeria | monocytogenes | 1/2b | + | List-NRC | 10/50 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2b | + | List-NRC | 10/68 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2b | + | List-NRC | 10/109 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2c | + | List-NRC | 10/48 | + | + | + | + |
| Listeria | monocytogenes | 1/2c | + | List-NRC | 10/16 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2c | + | List-NRC | 10/49 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2c | + | List-NRC | 10/58 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2c | + | List-NRC | 10/153 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2c | + | List-NRC | 10/160 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2c | + | List-NRC | 10/184 | nt | nt | + | + |
| Listeria | monocytogenes | 1/2c | + | List-NRC | 10/192 | nt | nt | + | + |
| Listeria | monocytogenes | 3a | + | List-NRC | 10/29 | + | + | + | + |
| Listeria | monocytogenes | 3a | + | List-NRC | 10/202 | nt | nt | + | + |
| Listeria | monocytogenes | 3a | + | List-NRC | 10/237 | nt | nt | + | + |
| Listeria | monocytogenes | 3a | + | List-NRC | 9/109 | nt | nt | + | + |
| Listeria | monocytogenes | 3a | + | List-NRC | 9/181 | nt | nt | + | + |
| Listeria | monocytogenes | 3a | + | List-NRC | 8/171 | nt | nt | + | + |
| Listeria | monocytogenes | 3b | + | List-NRC | 8/115 | + | + | + | + |
| Listeria | monocytogenes | 3b | + | List-NRC | Würzburg | + | + | + | + |
| Listeria | monocytogenes | 3c | + | List-NRC | 6/64 | + | + | + | + |
| Listeria | monocytogenes | 3c | + | List-NRC | 6/125 | + | + | + | + |
| Listeria | monocytogenes | 3c | + | List-NRC | 6/137 | nt | nt | + | + |
| Listeria | monocytogenes | 3c | + | List-NRC | 6/275 | nt | nt | + | + |
| Listeria | monocytogenes | 3c | + | List-NRC | 6/301 | nt | nt | + | + |
| Listeria | monocytogenes | 4a | + | List-NRC | 10/118 | + | + | + | + |
| Listeria | monocytogenes | 4a | + | List-NRC | ATCC 19114 | nt | nt | + | + |
| Listeria | monocytogenes | 4b | + | List-NRC | ATCC 51780 | + | + | + | + |
| Listeria | monocytogenes | 4b | + | List-NRC | 10/7 | + | + | + | + |
| Listeria | monocytogenes | 4b | + | List-NRC | 10/1 | nt | nt | + | + |
| Listeria | monocytogenes | 4b | + | List-NRC | 10/3 | nt | nt | + | + |
| Listeria | monocytogenes | 4b | + | List-NRC | 10/24 | nt | nt | + | + |
| Listeria | monocytogenes | 4b | + | List-NRC | 10/34 | nt | nt | + | + |
| Listeria | monocytogenes | 4b | + | List-NRC | 10/47 | nt | nt | + | + |
| Listeria | monocytogenes | 4c | + | EU-RL List | 09LEB41LM | + | + | + | + |
| Listeria | monocytogenes | 4d | + | List-NRC | 8/221 | + | + | + | + |
| Listeria | monocytogenes | 4d | + | List-NRC | 7/89 | nt | nt | + | + |
| Listeria | monocytogenes | 4d | + | List-NRC | 7/114 | nt | nt | + | + |
| Listeria | monocytogenes | 4d | + | List-NRC | 5/163 | nt | nt | + | + |
| Listeria | monocytogenes | 4e | + | List-NRC | 10/35 | + | + | + | + |
| Listeria | ivanovii | + | List-NRC | CIP 7842 | + | + | − | − | |
| Listeria | ivanovii | + | ILVO | LMG11388 | + | + | − | − | |
| Listeria | ivanovii | + | List-NRC | 06/124 | + | + | − | − | |
| Listeria | ivanovii | + | List-NRC | 06/129 | + | + | − | − | |
| Listeria | ivanovii | + | EU-RL List | TQA237 | + | + | − | − | |
| Listeria | ivanovii | + | EU-RL List | TQA238 | + | + | − | − | |
| Listeria | ivanovii | + | EU-RL List | 00CHPL02 | + | + | − | − | |
| Listeria | seeligeri | + | List-NRC | ATCC 35967 | + | + | − | − | |
| Listeria | seeligeri | + | ILVO | MB43:LMG16764 | + | + | − | − | |
| Listeria | seeligeri | + | EU-RL List | TQA231 | + | + | − | − | |
| Listeria | seeligeri | + | EU-RL List | TQA232 | + | + | − | − | |
| Listeria | welshimeri | + | List-NRC | ATCC 35897 | + | + | − | − | |
| Listeria | welshimeri | + | List-NRC | 06/102 | + | + | − | − | |
| Listeria | welshimeri | + | List-NRC | 06/229 | + | + | nt | nt | |
| Listeria | welshimeri | + | List-NRC | 04/341 | + | + | nt | nt | |
| Listeria | welshimeri | + | EU-RL List | 02CHPL153 | + | + | nt | nt | |
| Listeria | welshimeri | + | EU-RL List | 02CHPL154 | + | + | nt | nt | |
| Listeria | welshimeri | + | EU-RL List | 03CHPL91 | + | + | nt | nt | |
| Listeria | welshimeri | + | EU-RL List | TQA230 | + | + | nt | nt | |
| Listeria | innocua | + | EU-RL List | 03CHPL98 | + | + | nt | nt | |
| Listeria | innocua | + | List-NRC | CIP 8011 | + | + | − | − | |
| Listeria | innocua | + | ILVO | MB176(T) = FML2011 | + | + | − | − | |
| Listeria | innocua | + | List-NRC | 10/85 | + | + | nt | nt | |
| Listeria | innocua | + | List-NRC | 10/101 | + | + | nt | nt | |
| Listeria | innocua | + | List-NRC | 10/122 | + | + | nt | nt | |
| Listeria | innocua | + | List-NRC | 09/158 | + | + | nt | nt | |
| Listeria | innocua | + | List-NRC | 09/221 | + | + | nt | nt | |
| Listeria | innocua | + | List-NRC | 09/291 | + | + | nt | nt | |
| Listeria | innocua | + | List-NRC | 08/147 | + | + | nt | nt | |
| Listeria | innocua | + | List-NRC | 07/92 | + | + | nt | nt | |
| Listeria | innocua | + | List-NRC | 06/237 | + | + | nt | nt | |
| Listeria | innocua | + | IPH-FP | TIAC 706 | + | + | nt | nt | |
| Listeria | grayi | + | List-NRC | ATCC 25401 | − | − | − | − | |
| Listeria | grayi | + | ILVO | LMG16490 | − | − | − | − | |
| Aeromonas | hydrophila | − | IPH-CB | 6688 (M/2862 (EEQ 2003/2)) | − | − | − | − | |
| Bacillus | cereus | + | IPH-FP | ATCC 14579 | − | − | − | − | |
| Bacillus | circulans | + | IPH-FP | TIAC 100 | − | − | − | − | |
| Bacillus | lentus | + | IPH-FP | TIAC 101 | − | − | − | − | |
| Bacillus | lichiniformis | + | IPH-FP | TIAC 102 | − | − | − | − | |
| Bacillus | mycoides | + | IPH-FP | TIAC 97 | − | − | − | − | |
| Bacillus | sphaericus | + | IPH-FP | TIAC 104 | − | − | − | − | |
| Bacillus | subtillis | + | IPH-FP | TIAC 103 | − | − | − | − | |
| Bacillus | thuringiensis | + | IPH-FP | TIAC 96 | − | − | − | − | |
| Brevibacillus | borstelensis | + | IPH-FP | TIAC 099 | − | − | − | − | |
| Brochothrix | thermosphacta | + | IPH-FP | TIAC 400 | − | − | − | − | |
| Campylobacter | coli | − | IPH-FP | ATCC 33559 T | − | − | − | − | |
| Campylobacter | jejuni | − | IPH-FP | ATCC 33291 | − | − | − | − | |
| Campylobacter | lari | − | IPH-FP | TIAC 542 | − | − | − | − | |
| Carnobacterium | divergens | + | IPH-FP | Argentijns vlees B21 | − | − | − | − | |
| Citrobacter | freundii | − | IPH-FP | TIAC 554 | − | − | − | − | |
| Clostridium | perfingens | + | IPH-FP | ATCC 13124 T | − | − | − | − | |
| Enterobacter | cloacae | − | IPH-FP | TIAC 445 | − | − | − | − | |
| Enterococcus | faecalis | + | IPH-CB | ATCC 29212 | − | − | − | − | |
| Escherichia | coli | − | IPH-FP | ATCC 25922 | − | − | − | − | |
| Escherichia | coli | O157 | − | IPH-FP | EH 630 | − | − | − | − |
| Hafnia | alvei | − | IPH-CB | 7186 | − | − | − | − | |
| Klebsiella | pneumoniae | − | IPH-FP | TIAC 446 | − | − | − | − | |
| Lactobacillus | acidophilus | + | IPH-FP | Argentijns vlees A19 | − | − | − | − | |
| Lactobacillus | brevis | + | IPH-FP | Argentijns vlees A53 | − | − | − | − | |
| Lactobacillus | curvatus | + | IPH-FP | Argentijns vlees A1 | − | − | − | − | |
| Lactobacillus | delbrucki | + | IPH-FP | Argentijns vlees B17 | − | − | − | − | |
| Lactobacillus | plantarum | + | IPH-FP | Argentijns vlees B34 | − | − | − | − | |
| Lactococcus | lactis lactis | + | IPH-FP | Argentijns vlees A31 | − | − | − | − | |
| Leuconostoc | citreum | + | IPH-FP | Argentijns vlees B24 | − | − | − | − | |
| Leuconostoc | mesenteroides | + | IPH-FP | Argentijns vlees B6 | − | − | − | − | |
| Paenibacillus | polymyxa | + | IPH-FP | TIAC 105 | − | − | − | − | |
| Proteus | vulgaris | − | IPH-CB | 6223 (M/654) (EEQ 1996/3) | − | − | − | − | |
| Pseudomonas | aeruginosa | − | IPH-FP | LMG 6395 | − | − | − | − | |
| Salmonella | enterica enterica | Enteritidis | − | Salm-NRC | H, VI, 6, 32 | − | − | − | − |
| Salmonella | enterica enterica | Thyphimurium | − | Salm-NRC | H, II, 32, 32 | − | − | − | − |
| Serratia | marcescens | − | IPH-CB | 7015 | − | − | − | − | |
| Shigella | sonneï | − | Salm-NRC | 10-03865 | − | − | − | − | |
| Staphylococcus | aureus | + | IPH-FP | ATCC 25923 | − | − | − | − | |
| Staphylococcus | epidermidis | + | IPH-FP | TIAC 367 | − | − | − | − | |
| Staphylococcus | pisciferm | + | IPH-FP | TIAC 364 | − | − | − | − | |
| Streptococcus | feacales | + | IPH-FP | TIAC 300 | − | − | − | − | |
| Vibrio | parahaemoliticus | − | IPH-FP | TIAC 610 | − | − | − | − | |
| Yersinia | enterocolitica | − | IPH-FP | LMG 15558 | − | − | − | − | |
| Aspergillus | fumigatus | na | IPH-MA | BCCM/IHEM 19436 | − | − | − | − | |
| Cladosporium | sphaerospermum | na | IPH-MA | BCCM/IHEM 24474 | − | − | − | − | |
| Saccharomyces | cerevisiae | na | IPH-MA | BCCM/IHEM 3961 | − | − | − | − | |
| Candida | parapsilosis | na | IPH-MA | BCCM/IHEM 6478 | − | − | − | − | |
| Hepatitis A Virus | na | IPH-FP | 27 (WZ) | − | − | − | − | ||
| Norovirus | na | IPH-FP | 2593 | − | − | − | − | ||
| No template control | na | na | − | − | − | − | |||
+ there is an amplification and a T m value similar for all corresponding strains, − no amplification, List-NRC Belgian Listeria National Reference Centre, rue Juliette Wytsmanstraat 14, 1050 Brussels, Belgium, EU-RL List EU-RL Listeria monocytogenes, 23 avenue du Général de Gaulle, 94706 Maisons-Alfort cedex, France, ILVO Instituut voor Landbouw- en Visserijonderzoek, Technology & Food Science Unit, Food Safety, Product Quality and Innovation, and Business Unit and Service Centre, Brusselsesteenweg 370, 9090 Melle, Belgium, IPH-FP Scientific Institute of Public Health, Food Pathogens Laboratory, rue Juliette Wytsmanstraat 14, 1050 Brussels, Belgium, IPH-CB Scientific Institute of Public Health, Clinical Biology, rue Juliette Wytsmanstraat 14, 1050 Brussels, Belgium, Salm-NRC Belgian Salmonella and Shigella National Reference Centre, rue Juliette Wytsmanstraat 14, 1050 Brussels, Belgium, IPH-MA Mycology and Aerobiology, Scientific Institute of Public Health, rue Juliette Wytsmanstraat 14, 1050 Brussels, Belgium, nt not tested, na not applicable