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. 2012 Oct 20;97(9):4021–4037. doi: 10.1007/s00253-012-4477-2

Table 4.

Repeatability of the Cq values at the LOD for all Listeria SYBR®Green qPCR assays. The results are based on 36 repetitions with genomic targets as DNA template

Repeatability calculation on C q values at the LOD
L. monocytogenes 1/2a (ATCC 51772) L. monocytogenes 4b (ATCC 51777) Listeria ivanovii (LMG 11388)
RSDr (%) r RSDr (%) r RSDr (%) r
iap-50-deg 4.9 4.8 na na 2 1.9
prs-2-deg 2.4 2.2 na na 2.3 2.1
hlyA-177 3.8 3.6 4.1 3.8 na na
hlyA-146-deg-tronc 1.6 1.5 2.9 2.8 na na

na not applicable, LOD limit of detection