Table 5.
Repeatability of the Tm values for all Listeria SYBR®Green qPCR assays. The results are based on all Tm values coupled with amplification (Cq≠40)
| Repeatability calculation on T m value | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| L. monocytogenes 1/2a (ATCC 51772) | L. monocytogenes 4b (ATCC 51777) | Listeria ivanovii (LMG 11388) | |||||||
| T m value ± SD (°C) | RSDr (%) | r | T m value ± SD (°C) | RSDr (%) | r | T m value ± SD (°C) | RSDr (%) | r | |
| iap-50-deg | 76.8 ± 0.4 | 0.6 | 1.2 | nt | na | na | 75.9 ± 0.5 | 0.6 | 1.3 |
| prs-2-deg | 71.3 ± 0.2 | 0.2 | 0.5 | nt | na | na | 71.1 ± 0.3 | 0.4 | 0.7 |
| hlyA-177 | 74.3 ± 0.2 | 0.3 | 0.6 | 73.7 ± 0.3 | 0.4 | 0.9 | nt | na | na |
| hlyA-146-deg-tronc | 74 ± 0.4 | 0.6 | 1.2 | 74.5 ± 0.3 | 0.4 | 0.9 | nt | na | na |
nt not tested, na not applicable