. 2012 Oct 20;97(9):4021–4037. doi: 10.1007/s00253-012-4477-2

Table 6.

Reproducibility test of the four SYBR®Green Listeria spp. and L. monocytogenes detection assays

		Sample	L. mono 1/2b (ATCC 51780)	L. mono 4d (BCRL: 7/89)	L. mono 4b (BCRL: 10/47)	L. mono 3b (Würburg)	L. mono 4a (ATCC 19114)	L. mono 1/2c (BCRL: 10/49)	L. mono 3a (BCRL: 9/109)	L. mono 4c (EU-RL: 09LEB41)	Uncertainty (99 %)
		Copy number	20	50	100	200	50	20	20	5
iap-50-deg	C _q	Average Lab 1	29.58	28.16	27.45	26.80	28.46	29.57	29.49	31.59	1.44
		Average Lab 2	29.15	27.37	27.65	26.67	27.29	28.45	28.52	32.03
		RSD_R (%)	1.03	2.03	0.50	0.35	2.98	2.72	2.35	0.96
	T _m	Average Lab 1	77.00	77.00	76.50	76.75	76.50	77.00	77.00	76.50	1.21
		Average Lab 2	76.35	76.35	75.50	76.05	76.05	76.50	76.50	76.05
		RSD_R (%)	0.60	0.60	0.93	0.65	0.42	0.46	0.46	0.42
prs-2-deg	C _q	Average Lab 1	28.38	26.62	26.04	25.44	27.31	28.36	28.45	30.03	0.95
		Average Lab 2	27.91	26.48	25.42	25.72	26.46	28.23	27.93	30.56
		RSD_R (%)	1.19	0.35	1.70	0.78	2.24	0.35	1.31	1.24
	T _m	Average Lab 1	71.75	71.75	71.50	72.00	71.75	71.50	71.75	71.50	0.46
		Average Lab 2	71.60	71.60	71.45	71.45	71.60	71.60	71.45	71.45
		RSD_R (%)	0.15	0.15	0.05	0.54	0.15	0.10	0.30	0.05
hlyA-177	C _q	Average Lab 1	30.28	28.33	27.51	29.32	28.83	30.05	29.57	31.88	1.30
		Average Lab 2	29.16	27.63	27.60	28.53	28.29	29.05	29.74	31.61
		RSD_R (%)	2.66	1.77	0.23	1.92	1.33	2.41	0.40	0.61
	T _m	Average Lab 1	74.00	74.00	74.00	74.00	73.50	74.00	74.00	74.00	0.88
		Average Lab 2	73.75	73.75	73.40	73.40	73.20	73.75	73.40	73.40
		RSD_R (%)	0.24	0.24	0.58	0.58	0.29	0.24	0.58	0.58
hlyA-146-deg-tronc	C _q	Average Lab 1	29.90	28.14	27.65	27.20	28.95	30.37	30.53	32.65	3.72
		Average Lab 2	27.65	26.03	26.23	25.70	26.75	28.46	27.96	31.28
		RSD_R (%)	5.53	5.50	3.74	4.02	5.58	4.61	6.19	3.02
	T _m	Average Lab 1	75.00	75.00	74.75	75.00	73.50	73.75	74.00	74.75	1.42
		Average Lab 2	74.50	74.35	74.05	74.05	73.05	73.20	73.05	73.75
		RSD_R (%)	0.47	0.62	0.67	0.90	0.43	0.53	0.91	0.95