Table 8.

Matrix of targets amplification with the four SYBR®Green qPCR assays: experimental verification

Sample Name	Detector	iap-50-deg		prs-2-deg		hlyA-177		hlyA-146-deg-tronc
L. monocytogenes 4b	C q	23.45	22.99	21.66	21.57	22.95	22.75	22.48	22.58
	T m	76.9	76.9	71.7	71.7	74.1	74.1	74.7	74.7
	Conclusion	Listeria spp. present		Listeria spp. present		L. monocytogenes present		L. monocytogenes present
	Average C q	22.55
	St dev C q	0.65
L. ivanovii	C q	23.69	23.29	22.23	22.11	Und	Und	34.67	38.67
	T m	75.9	76.3	71.7	71.7	72.6	74.7	74.7	71.7
	Conclusion	Listeria spp. present		Listeria spp. present		Below LOD		Below LOD
	Average C q	22.83				na
	St dev C q	0.78
L. seeligeri	C q	26.73	26.09	25.02	25.48	34.72	37.91	Und	Und
	T m	75.9	75.9	71.7	71.7	74.1	74.1	73	72.1
	Conclusion	Listeria spp. present		Listeria spp. present		Below LOD		Below LOD
	Average Cq	25.83				na
	St dev C q	0.74
L. grayi	C q	Und	35.02	34.17	Und	Und	35.86	Und	Und
	T m	70	74.4	71.5	71.7	74.4	74.4	70	70
	Conclusion	Below LOD		Below LOD		Below LOD		Below LOD