Wing kinematics and FRET of Drosophila A-IFM at rest (R) and in flight during optomotor yaw response. Traces show left stroke amplitude (SAL, red), right stroke amplitude (SAR, blue), stroke frequency (FREQ, black), mean stroke amplitude of both wings (SA, blue), left-minus-right stroke amplitude difference (ΔSA, black), the corresponding outputs of total mass-specific A-IFM mechanical power (P*mech, blue) and power difference (ΔP*mech, black) and the changes in mean FRET of A-IFM fibres, scored from thoracic muscle attachment sites for left and right dorsoventral muscle (DVM46,48, black) and dorso-longitudinal muscle (DLM45a–c, blue). The traces below show left-minus-right difference (Δ) in FRET of selected A-IFM fibres and the calcium activation of the synchronous tergo-trochanter muscle (TTM) of the animal's middle leg. Stimulus velocity of the visual pattern is shown at the bottom, where positive (negative) values indicate optomotor yaw stimulation to the right (left). The traces are typical of the measured animals and were simultaneously recorded in a single fly. Zero is indicated by a dotted line.