Table 1.

Calcium activation slopes and fibre-specific comparison of asynchronous indirect flight muscle (A-IFM) during flight of transgene Cam2.1 Drosophila. Slopes are calculated from linear regressions to binned mean FRET values and A-IFM mass-specific power estimates. Note that DLM45a–c employs only two motor neurons for three muscle fibres [57]. Data were recorded from flight sequences of 25 flies, where each sequence was approximately 5 min. Maximum mean %ΔFRET saturates between 120 and 140 W kg⁻¹ muscle (mean±s.d.). R, correlation coefficient; p, probability; n.a., not applicable; TTM, tergo-trochanter muscle.

muscle	fibres	%ΔFRET maximum	%ΔFRET W−1 ga	R2a	pslopea	%ΔFRET W−1 ga,b	%ΔFRET W−1 gc	R2c	pslopec	yaw/lift slope ratio (%)	psloped
DLM45a–c	2	17.9±1.39	91.7	0.95	<0.001	45.9	28.1	0.94	<0.001	30.6	<0.001
DLM45d–f	3	17.0±1.14	85.3	0.95	<0.001	28.4	14.6	0.80	<0.002	17.1	0.001
DVM46,48	4	19.2±1.70	91.7	0.96	<0.001	22.9	20.1	0.89	<0.001	21.9	<0.001
DVM47a–c	3	17.0±1.51	81.3	0.97	<0.001	27.1	44.0	0.88	<0.001	54.1	0.004
TTM	n.a.	4.09±0.62	26.9	0.81	<0.001	n.a.	−5.8	0.66	0.008	−21.6	0.003

^aLinear regression slope during optomotor lift stimulation at flight power ranging from 20 to 120 W kg⁻¹ muscle mass.

^bMotor neuron-specific value.

^cRegression slope during yaw stimulation for power ranging from −20 to 20 W kg⁻¹ muscle mass.

^dt-test on slope difference between lift and yaw stimulation.