Figure 3. Spontaneous and GABA-evoked currents of RBCs in the grm6-TeNT retina.
(a) Spontaneous GABAergic currents recorded in grm6-TeNT and wildtype (WT) RBCs at P11–13 and P30. (b) Quantification revealed no significant differences in either the mean frequency or mean amplitude of GABAergic sIPSCs from grm6-TeNT RBCs as compared to WT. (c) Example traces showing chloride-mediated outward currents in RBCs evoked by AMPA puffs in grm6-TeNT and WT RBCs at both ages. The GABAC component was isolated by application of TPMPA, and residual GABAA-mediated current blocked by SR95531. Scatter plots show that the mean charge transfer of the total, GABAA-mediated and GABAC-mediated currents are comparable in grm6-TeNT RBCs and WT at P11–13 (d) and P30 (e). Numbers in brackets in b, d, and e represents number of cells. Error bars represent S.E.M.