Figure 5. GABAA and GABAC receptor-mediated currents from P30 RBCs in GAD1KO retina.
(a) Example traces of P30 GAD1KO and littermate control (ctr) RBC responses to GABA puffs. TPMPA was applied to isolate the GABAA-mediated component. Further addition of SR95531 blocked all evoked response. (b) Scatter plots show that mean peak amplitude and charge of GABAA-mediated currents in GAD1KO RBCs are significantly reduced as compared to ctr. (c) Application of SR95531 alone isolates the GABAC-mediated component of evoked responses in RBCs. (d) Quantification of GABAC-mediated currents indicates a significant reduction of the mean charge in GAD1KO RBCs as compared to ctr. However, the mean amplitude remained unchanged. Numbers in brackets in b and d represents number of cells. Asterisks mark significant difference. Error bars represent S.E.M.