Figure 2. Models and current traces show the 8.0 μM SC₄ binding with α-HL.

(a) SC₄ were driven into the trans side of α-HL and induced the close-states of α-HL by binding with the positive-charged residues inside the stem. (b) The obtained raw data by the addition of SC₄ to the trans chamber at the holding potential of −60 mV (top), −70 mV (middle) and −80 mV (bottom). (c) The illustration of the inhibition of an α-HL in the presence of SC₄ at the cis chamber. (d) The raw data for the current traces recorded after SC₄ was driven into the cis side of an α-HL at the holding potential of +90 mV (top), +100 mV (middle) and +110 mV (bottom).