Figure 4. Analysis of irreversible close-states of α-HL in the presence of 8.0 μM SC₄ at the trans compartment.

(a) Representative current traces of the irreversible inhibition at the holding potential of −100 mV and the repulsive potential of −40 mV. (b) The irreversible inhibitions at the holding potential from −70 mV to −140 mV fall into two populations, PIV and PV, after treated with the repulsive potential. The majority of the irreversible inhibitions located at PV demonstrate that the irreversible close-state results from the complete closure of the channel.