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. 2013 Apr 16;3:1661. doi: 10.1038/srep01661

Figure 3. Xentry-mediated delivery of antibody and siRNA cargoes into cells.

Figure 3

(a) Uptake of Xentry-conjugated and unconjugated FITC-labelled rabbit IgG by HepG2 cells. Cell nuclei were stained blue with DAPI. (b) Representative cell showing staining of tubulin by a Cy3-labelled anti-β-tubulin antibody delivered to HepG2 cells by Xentry. HepG2 cells were incubated with the Xentry- anti-β-tubulin antibody conjugate for 1 h, washed, then incubated and photographed 24 h later. For comparison, a representative fixed and permeabilized cell is shown which has been stained by the free anti-β-tubulin antibody. The unconjugated anti-β-tubulin antibody could not penetrate and stain non-permeabilized HepG2 cells. (c) The abilities of a Xentry-conjugated and unconjugated anti-B-raf antibody to induce the apoptosis of WM-266-4 melanoma cells, as evidenced by staining of cells with annexin-V fluos (green). (d) A Xentry-KALA fusion peptide delivers an siRNA directed against B-raf transcripts into WM-266-4 melanoma cells, causing cell apoptosis as evidenced by staining of cells with annexin-V fluos (green). For comparison, the anti-B-raf siRNA was transfected into cells using the PolyMag agent. The siRNA was omitted from controls. Scale bar, 50 μm.