Table III.

The Exponential Residency Times within the FA Zone (τ_in) for the Full-Length Rac1 and Tail Except for the Molecules Immobilized within the FA Zone during the Entire Observation Period

Molecules	τin (s)	nmol	Total observation period (s)
WT	0.24	106	43
G12V	0.81	137	119
T17N	0.28	183	81
Tail	0.14	277	85

The analysis was performed for the molecules that remained within the FA zone longer than 11 frames (10 steps or 165 ms in trajectories). These values were corrected for the photobleaching lifetime of the TMR (14.2 s). For the derivation of the residency times, see Supporting Information, Fig. S1.