Table IV.

Percentages of Molecules that Entered FA Zones via Lateral Diffusion after Binding to the PM Outside FA Zones Or Directly Arrived in the FA Zone from the Cytoplasm

Molecules	Via lateral diffusion on the PM (%)	Direct recruitment from the cytoplasm (%)
WT	70.2 ± 4.9	29.8 ± 4.9
G12V	68.1 ± 4.9	31.9 ± 4.9
T17N	75.2 ± 3.7	24.8 ± 3.7
Tail	78.2 ± 0.3	21.8 ± 0.3
αPixa	3.8 ± 1.5	96.2 ± 1.5
βPixa	5.0 ± 0.6	95.0 ± 0.6

^aValues for αPix and βPix include those immobilized within the area between 0 and 200 nm from the edge of the FA zone (see the first bin in the histograms shown in Fig. 2) due to the following reason. Mostly due to noise (limited localization accuracies of single molecules), many molecules that directly arrived in this area and immobilized there apparently move back and forth between this area and the area between −200 nm and 0 nm from the FA edge. This distorts the count of molecules that entered the FA zone directly from the cytoplasm and via lateral diffusion on the PM.