Skip to main content
. Author manuscript; available in PMC: 2013 Apr 16.
Published in final edited form as: Circulation. 2000 Feb 15;101(6):660–667. doi: 10.1161/01.cir.101.6.660

Figure 2.

Figure 2

IGF-1 activates Akt in a wortmannin-sensitive manner. A, IGF-1 activates Akt kinase activity. After serum starvation, cardiomyocytes were stimulated with IGF-1 (50 ng/mL) for 15 minutes. Cell lysates were prepared and immunoprecipitated with an antibody specific for Akt in absence or presence of competitor peptides (Comp). Immunoprecipitated kinase activity was measured with histone H2B as a substrate. B, Activation of Akt by IGF-1 is blocked by wortmannin. Cells were pretreated with wortmannin (Wort, 200 nmol/L) and stimulated with IGF-1 (50 ng/mL) for 15 minutes. Akt kinase activity was measured as described in A.