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. Author manuscript; available in PMC: 2013 Apr 16.
Published in final edited form as: Circulation. 2000 Feb 15;101(6):660–667. doi: 10.1161/01.cir.101.6.660

Figure 3.

Figure 3

Adenovirus constructs expressing Akt molecules. A, Structures of replication-defective adenovirus vectors expressing wild-type (wtAkt), dominant-negative (dnAkt), or constitutively active (myrAkt) Akt fused to HA. B, Adenoviral constructs express comparable levels of transgene proteins. Cardiomyocytes were transfected with adenovirus vector at MOI 25. Cell lysates were prepared and protein concentrations determined. Proteins (10 μg) were immunoblotted with anti-HA antibody. Adenoviral vector encoding β-galactosidase (β-gal) was used as a negative control. C, Kinase activities of adenovirally encoded Akt proteins. Cardiac myocytes were infected with adenovirus vectors and stimulated with IGF-1 (50 ng/mL) for 15 minutes. Cell lysates were immunoprecipitated with anti-HA antibody and assayed for kinase activities.