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. Author manuscript; available in PMC: 2013 Apr 16.
Published in final edited form as: Circulation. 2000 Feb 15;101(6):660–667. doi: 10.1161/01.cir.101.6.660

Figure 5.

Figure 5

Akt is necessary and sufficient for IGF-1–mediated myocyte survival. A, Representative image of TUNEL (TNL)-positive cardiac myocytes in culture. Cardiac myocytes were cultured in absence of serum for 48 hours. Cells were stained with Hoechst, TUNEL, and anti–α-sarcomeric actin antibody. B, Effects of Akt-expressing adenovirus vectors on cardiac myocyte survival. Cultured cells were infected with mock (no virus) or adenovirus vectors expressing β-galactosidase, wild-type, dominant-negative, or constitutively active Akt (β-gal, wtAkt, dnAkt, and myrAkt, respectively). After infection, cells were cultured in presence of indicated concentrations of IGF-1 for 48 hours. Myocyte viability was assessed by staining with Hoechst and TUNEL. Cultured cells were identified as cardiomyocytes by staining with anti–α-sarcomeric actin antibody. Data are shown as mean±SEM (n=4).