Control and patient samples and SDS-PAGE standards were labeled with Cy5-, Cy3-, and Cy2-, respectively, and separated by 2D-DIGE. Individual images of labeled proteins were obtained using a Typhoon 9410 scanner and compared using DeCyder 2D software (v7.0). Top to bottom: Control and patient samples individually, superimposed images of control and patient, and western blot of the 2D-DIGE using anti-human Bβ chain antibody (59D8). The Bβ chain pIs are indicated in the patient image.