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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Thromb Haemost. 2012 Jul 26;108(3):516–526. doi: 10.1160/TH12-05-0355

Figure 4. Whole and defibrinated patient plasmas demonstrate abnormal thrombin generation that can be recapitulated by raising the FVIII level in NPP.

Figure 4

Figure 4

Figure 4

Thrombin generation was initiated by adding 4 μM phospholipid and 1 pM TF to whole (A) or defibrinated (B, C) plasmas. CAT detects higher thrombin generation in the presence of fibrinogen than in its absence because fibrin-bound thrombin is protected from inhibition by antithrombin III but can still cleave fluorogenic substrate (3, 56, 57). A, B) Symbols are: NPP (○), patient plasma (●). C) Thrombin generation was initiated by adding 4 μM phospholipid and 1 pM TF to defibrinated NPP (○), defibrinated patient plasma (●), and defibrinated NPP plus FVIII (✞, 225%, final). Data show mean and standard deviations from experiments performed in triplicate in three independent assays for whole plasmas, and in triplicate in two to five independent assays for defibrinated plasmas.