Table 3. Thrombin generation parameters in whole and defibrinated plasmas.
Whole Plasmaa | Defibrinated Plasmab | ||||
---|---|---|---|---|---|
NPP | Patient | NPP | Patient | NPP plus FVIII | |
Lag Time (min) | 4.4 ± 0.2 | 4.5 ± 0.2 | 3.7 ± 0.4 | 4.0 ± 0.4 | 3.8 ± 0.6 |
Time to peak (min) | 10.2 ± 0.2 | 9.4 ± 0.2* | 10.4 ± 2.0 | 9.0 ± 0.3 | 7.7 ± 0.5 |
Peak thrombin (nM) | 183 ± 16 | 267 ± 20* | 107 ± 27 | 158 ± 14# | 181 ± 45# |
ETPc (nM.min) | 2052 ± 141 | 2343 ± 162 | 1066 ± 216 | 1244 ± 74 | 1146 ± 119 |
Experiments were triggered with 1 pM TF and 4 μM phospholipids.
Mean ± standard deviation from three independent experiments from two separate blood draws, performed in triplicate
Mean ± standard deviation from two to five independent experiments performed in triplicate
ETP, endogenous thrombin potential
p<0.05 versus whole NPP
p<0.01 versus defibrinated NPP