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. Author manuscript; available in PMC: 2013 Apr 16.
Published in final edited form as: Eur J Pharmacol. 2008 Feb 19;586(0):179–188. doi: 10.1016/j.ejphar.2008.02.035

Figure 4.

Figure 4

Effect of opioid and excitatory amino acid receptor blockade on withdrawal jumping after acute and chronic heroin treatment. ACUTE (left figure): mice were injected with a single heroin dose (50 mg/kg). CHRONIC (right figure): mice were injected t.i.d. for three days with escalating heroin doses (10, 20, and 40 mg/kg on treatment Days 1, 2, and 3, respectively) and a final 40 mg/kg heroin dose on Day 4. All mice were also injected with one of the following antagonists: BNTX, NTB, nor-BNI (δ1, δ2, and κ opioid receptor antagonists, respectively), MK-801 and LY293558 (NMDA and AMPA receptor antagonists, respectively: see text for antagonist doses and injection schedule). MK-801 and LY293558 were also delivered by continuous infusion during acute heroin treatment. Withdrawal was precipitated by naloxone (50 mg/kg) injection 2 and 1 h after completing acute and chronic heroin treatment, respectively. Control mice in both treatment paradigms were injected with saline instead of antagonist. Significant differences from control group are indicated (*P <0.05).