Fig. 2.
Spontaneous inhibitory synaptic currents in leaner PCs are more frequent and have larger amplitude than in wild-type cells. a, b Voltage-clamp recordings of sIPSCs in the presence of kynurenic acid at different holding potentials. Mean sIPSC amplitude varies linearly with holding potential and reverses near ECl. c, d Comparison between sIPSCs in WT and leaner PCs under control conditions (upper set of three traces) and in the presence of picrotoxin (200 µM, lower traces). Picrotoxin abolishes the majority of the spontaneous postsynaptic currents. e, f Normalized sIPSC amplitude histograms from the cells shown in c, d. Inset to f shows two examples of sIPSC bursts (from first and second traces of d) on expanded scale. g, h Normalized interevent interval (IEI) histograms from the cells are shown in c, d. Smooth traces show exponential fits to the respective distributions. i Summary data comparing sIPSC frequency, amplitude, and burst frequency in WT and leaner PCs. *P<0.05