Fig. 6.

Effect of thapsigargin on spontaneous IPSCs in WT and leaner PCs. a, b Representative recordings of sIPSCs from control WT and leaner PCs. c, d Representative recordings of sIPSCs from another group of cells that was pretreated for 10–15 min with thapsigargin (10 µM) followed by washout in the recording chamber. e Summary of the effects of thapsigargin on mean sIPSC frequency, amplitude, and burst frequency. Note that thapsigargin specifically reduces mean sIPSC frequency, amplitude, and burst frequency in leaner PCs, but has little effect on WT cells. Moreover, after treatment with thapsigargin, there was little or no difference between WT and leaner PCs in terms of sIPSC frequency, amplitude, and burst frequency. Dotted bars indicate mean values of the respective parameters under control conditions (without thapsigargin treatment). All recordings were made in the presence of kynurenic acid (5 mM)