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. Author manuscript; available in PMC: 2013 Apr 16.
Published in final edited form as: J Immunol Methods. 2006 Dec 29;320(0):18–29. doi: 10.1016/j.jim.2006.11.010

Figure 1.

Figure 1

Figure 1

Figure 1

Titration of cell numbers per well. Dilutions of cryopreserved peripheral blood mononuclear cells (PBMC) ranging from 0 – 8 × 105 cells/well were obtained from HIV-infected donor NB 001 and stimulated in triplicate with 2 peptides containing a major histocompatibility complex class I restricted HIV epitope identified in previous experiments. The mean total of IL-2 (Panel A), IFN-γ (Panel B) and PBMC secreting both cytokines (Panel C) spot forming cells per million PBMC are graphed for each cell concentration. Error bars represent standard deviation of the mean. Dotted lines represent cutoff values for a positive response for each cytokine as described in the Methods section.