Table 1. Effect of cell per well input on SFC/106 PBMC.
Cryopreserved peripheral blood mononuclear cells (PBMC) obtained from 4 HIV-infected donors were stimulated in triplicate with 2 peptides each containing a major histocompatibility complex class I restricted HIV epitope identified in previous experiments.
| Cytokine measured | Patient ID | Peptides testeda | Cell input (x10)
|
||
|---|---|---|---|---|---|
| 1.5 | 2.0 | 3.0 | |||
|
| |||||
| IL-2 | HDM 011 | Nef 5164 | 797.8 ± 76.9 b | 933.3 ± 64.1 | 930.0 ± 58.4 |
| Env 8957 | 551.1 ± 184.9 | 562.2 ± 7.63 | 506.7 ± 43.6 | ||
| HTM 375 | Pol 5543 | 111.1 ± 30.8 | 161.7 ± 39.7 | 245.6 ± 28.5 | |
| Nef 5186 | 228.9 ± 37.1 | 242.0 ± 32.8 | 314.4 ± 6.9 | ||
| LTNP 009 | Pol 5623 | 288.9 ± 36.7 | 346.7 ± 57.5 | 340 ± 28.0 | |
| Vif 6022 | 73.3 ± 11.5 | 133.3 ± 16.1 | 103.3 ± 33.6 | ||
| NB 001 | Gag 7959 | 482.2 ± 64.8 | 586.7 ± 90.2 | 551.1 ± 33.3 | |
| Pol 4296 | 168.9 ± 34.2 | 198.3 ± 2.9 | 210.0 ± 43.4 | ||
|
| |||||
| IFN-γ | HDM 011 | Nef 5164 | 1735.6 ± 83.4 | 1825.2 ± 94.9 | 1628.9 ± 62.0 |
| Env 8957 | 1375.6 ± 524.5 | 1420.9 ± 65.1 | 1063.3 ± 34.0 | ||
| HTM 375 | Pol 5543 | 364.4 ± 63.6 | 455.0 ± 28.9 | 584.4 ± 18.6 | |
| Nef 5186 | 937.8 ± 54.6 | 863.9 ± 71.5 | 966.7 ± 38.3 | ||
| LTNP 009 | Pol 5623 | 591.1 ± 65.7 | 750 ± 70.1 | 728.9 ± 24.0 | |
| Vif 6022 | 177.8 ± 40.0 | 281.7 ± 10.0 | 246.7 ± 16.8 | ||
| NB 001 | Gag 7959 | 486.7 ± 57.5 | 628.3 ± 67.1 | 597.8 ± 57.0 | |
| Pol 4296 | 164.4 ± 40.2 | 188.3 ± 2.9 | 200.0 ± 36.9 | ||
|
| |||||
| Dual | HDM 011 | Nef 5164 | 624.4 ± 76.7 | 779.3 ± 43.8 | 828.9 ± 45.1 |
| Env 8957 | 428.9 ± 165.6 | 457.4 ± 22.5 | 425.6 ± 40.6 | ||
| HTM 375 | Pol 5543 | 73.3 ± 13.3 | 103.3 ± 26.5 | 161.1 ± 22.2 | |
| Nef 5186 | 171.1 ± 33.6 | 173.1 ± 21.0 | 234.4 ± 1.9 | ||
| LTNP 009 | Pol 5623 | 128.9 ± 10.2 | 200.0 ± 27.5 | 183.3 ± 20.1 | |
| Vif 6022 | 40.0 ± 13.3 | 85.0 ± 20.2 | 65.6 ± 23.3 | ||
| NB 001 | Gag 7959 | 266.7 ± 30.1 | 336.7 ± 51.3 | 331.1 ± 20.4 | |
| Pol 4296 | 86.7 ± 19.2 | 81.6 ± 10.4 | 87.7 ± 25.0 | ||
a
Input number of cells per well.
b
Mean ± standard deviation of spot forming cells generated in response to each stimulus normalized to spot forming cells per million PBMC (SFC/106 PBMC).
c
Stimulatory peptides. Codes refer to catalog numbers assigned to individual peptides available from the National Institutes of Health AIDS Research and Reference Reagent Program.