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. Author manuscript; available in PMC: 2013 Apr 16.
Published in final edited form as: J Invest Dermatol. 2012 May 10;132(9):2255–2262. doi: 10.1038/jid.2012.135

Figure 2.

Figure 2

Regulation of MC1R gene expression by α-MSH, ASIP, HBD3 and UV. Melanocytes were maintained in medium lacking TPA and bovine pituitary extract overnight, then treated with 0, 1 nM α-MSH, 1 μM forskolin, 100 nM HBD3 or ASIP, or irradiated with 75 or 105 mJ/cm2 UV. Total RNA was isolated 8 hours after treatment, and equal amounts of RNA from each group were analyzed by qRT PCR. Similar results were obtained in 2 independent experiments using 2 different melanocyte strains. The data was normalized using GAPDH as a loading control and mean relative expression levels are presented +/− SEM.