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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Cell Mol Bioeng. 2013 Mar 2;6(1):26–37. doi: 10.1007/s12195-013-0274-y

Figure 2.

Figure 2

Actin breaking is myosin dependent. (A) Images of fluorescently labeled actin filaments in a flow cell with no myosin and 30 μM ATP obtained at 0 (left) and 30 minutes (right) after addition of actin. (B) Images of actin filaments during a myosin-based in vitro motility assay performed at 1 mM ATP obtained at 0 (left) and 1 minute (right) after addition of motility buffer. (C) Images of actin filaments during a myosin-based in vitro motility assay performed at 0.03 mM ATP obtained at 0 (left) and 1 (right) minute following addition of motility buffer.