Length dependence of the rate of actin filament breaking. (A) Histogram of actin filament break times, τbreak, measured during an in motility assay performed at 30 μM ATP for actin filaments with lengths (A) between 0 and 3.3 μm (n = 106), (B) between 3.3 and 6.6 μm (n = 151), and (C) greater than 6.6 μm (n = 54). Each histogram is fitted to a single exponential (solid line) to obtain the actin filament lifetime (τbreak). (D) Actin lifetimes for 0 – 3.3 μm, 3.3 – 6.6 μm, and > 6.6 μm length actin filaments are plotted.