Figure 7.
The effects of increasing myosin density on blebbistatin-inhibited V. Actin filament velocities (V) are plotted as a function of actin filament length (n = 120), where longer actin filaments interact with a higher number of myosin heads (N). N can be calculated from actin filament length as previously described (16). Points are shown in the absence (light gray) and presence (dark gray) of 50 μM blebbistatin. These data show that the inhibition of V by blebbistatin cannot be reversed by increasing myosin density.