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. 2013 Feb 1;133(1):79–89. doi: 10.1093/toxsci/kft019

Fig. 3.

Fig. 3.

MWCNT induces epithelial cell activation. SAEC were serum starved, exposed to DM control or 1.2 µg/ml MWCNT for 1, 6, or 24h, and lysed for analysis by SDS-PAGE. Whole-cell lysates were resolved on an 8% polyacrylamide gel and probed for (a) phosphotyrosine or (b) phosphothreonine. β-Actin was used as a loading control. (c) SAEC migration after MWCNT exposure was determined using a modified Boyden chamber. Migrated cells were stained with 0.1% crystal violet, eluted with acetic acid, and signal intensity read on a microplate reader. Data are presented as mean ± SEM of five assays. * Statistically significant at p ≤ 0.05.