Effects of DA storage depletion and reuptake transporter inhibition on MeHg-induced DA release. (A) Concentrations of extracellular DA from PC12 cells preincubated with 50nM reserpine for 60min, 1µM DMI for 15min, or vehicle in culture medium prior to cotreatment with 0µM (white bars) or 2µM (black bars) MeHg in HBS for 60min. * indicates a significant difference from 0µM MeHg-HBS (control) (p ≤ 0.05). # indicates a significant difference from 2µM MeHg-HBS (control) (p ≤ 0.05). (B) The percentage change of extracellular DA after treatment with MeHg in cells treated with HBS, 50nM reserpine, or 1µM DMI. * indicates a significant difference from HBS (p ≤ 0.05). Values are means ± SEM (n = 3, three replicates per n).