Table 1 .
MeHg (µM)a | Cell death (% of population) | |||
---|---|---|---|---|
Time (min) | ||||
15 | 30 | 60 | 120 | |
0 | 0.8±0.2b | 1.0±0.3 | 0.8±0.2 | 4.1±0.9 |
1 | 0.8±0.3 | 1.4±0.6 | 1.8±0.4 | 5.8±3.4 |
2 | 0.7±0.2 | 2.2±0.2 | 2.8±0.9 | 4.6±2.1 |
5 | 2.0±0.4 | 10.3±4.9 | 48.3±17.8* | 71.6±21.0* |
Notes. aPC12 cells were exposed to 0, 1, 2, or 5µM MeHg in HBS for 15, 30, 60, or 120min. Following toxicant treatment, cells were incubated with Hoechst 33342 and propidium iodide for 15min at 37°C prior to visualization and image acquisition. Viable cells had a blue-stained (Hoechst) nucleus, whereas nonviable cells had a low blue, high red (propidium iodide) fluorescence.
bValues are means ± SEM.
*The asterisk indicates a value significantly different from 0µM MeHg within time point (p < 0.05).