Fig. 1.

Inhibitors of canonical Wnt signaling inhibit prostatic bud number similar to TCDD. E14.5 male UGSs were cultured for 4 days in media containing 10nM DHT with vehicle, 1nM TCDD, recombinant DKK1 + DKK2 (500ng/ml each), or 10µM XAV-939 to inhibit canonical Wnt signaling. Buds were visualized by performing IHC specific for e-cadherin, an epithelium marker. The number of prostatic buds was determined by confocal microscopy. Arrowheads indicate areas where buds are present. U indicates urethra. Results are mean ± SE for at least four litter-independent samples per treatment. Asterisk indicates a significant decrease compared with control p < 0.05.