Fig. 1.
Emesis induction in mink corresponds to elevation in plasma DON. (A) Experimental design for DON-induced plasma PYY3–36 and 5-HT release; (B) mean cumulative emetic events (total retches and vomits) in mink following ip exposure to DON. Data are averages for both responders and nonresponders. The numbers of animals responding/tested at 15, 30, 60, and 120min were 6/16, 9/12, 8/8, and 4/4, respectively, for 0.1mg/kg bw DON group and 11/16, 12/12, 8/8, and 4/4, respectively, for 0.25mg/kg bw DON group. The mean latency time to onset of emesis for the 0.1 and 0.25mg/kg DON groups were 17±2 and 12±2min, respectively. (C) Kinetics of plasma DON concentration. Data represent mean ± SEM (n = 4 per group). A two-way ANOVA using Bonferroni t-test was used to assess significant differences in cumulative emetic events and kinetics of DON concentration in plasma. *p < 0.05 indicates statistically significant differences in emetic events or DON concentration compared with the control. ŧ p < 0.05 indicates a statistically significant difference in emetic events relative to the 0-min time point or significant difference in DON concentration relative to the 120-min time point within a given dose.