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. 2013 Feb 12;41(7):4207–4218. doi: 10.1093/nar/gkt083

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Inhibition of Pab-Pol D (exo⁺) by template strand uracil. (A1 and B1) Primer templates used for the experiments shown in panels A2 and B2, respectively. (A2) Extension of the 17/87 primer-template A1 (X = C or U) by Pab-Pol for the times indicated. The numbers under the gel lanes represent the total percentage of extended product. Reference oligodeoxynucleotides of 17, 33 and 87 bases are indicated by the arrows. (B2) Influence of burying uracil in double stranded DNA on extensions by Pab-Pol D. The image shows the products formed with the 17/87 primer-template B1 (X = T or U) with either the ‘masking strand’ present or absent. Each reaction shows a zero and 30-min time point. The numbers under the gel lanes show the total percentage of extended product. Reference oligodeoxynucleotides of 17, 57 and 87 bases are indicated by the arrows.