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Binding of Pfu-Pol D to primer templates containing T or U at the +4 template position (sequences given in Table 1). The polymerase was added to the hexachlorofluorescein-labelled primer templates and the increase in fluorescence anisotropy noted. The data were fitted to a 1:1 binding stoichiometry to give the titration curves shown. Binding constants are summarized in Table 1.
