Figure 4.
(A) The DNA duplexes including GC1, GC2, AT2 and GG2 were used in this study (squares represents the binding sites for the phenoxazone ring of ActD). (B) The effect of ActD at various concentrations on the ΔTm values of GC1, GC2, GG2 and AT2 (3 μM) in standard buffer. (C) The CD spectra of GC1, GC2, AT2 and GG2 (4 μM) in standard buffer alone and with 10 μM ActD are shown. The CD spectra of ActD–DNA complexes were obtained by subtracting out the spectra of ActD. (D) A sensorgram of the ActD–DNA interaction between immobilized hairpin DNA and the target ActD obtained by subtracting the reference control is shown. The concentration of the drug was 2 µM in 50 mM NaCl buffered with 20 mM Tris–HCl buffer at pH 7.3.