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. 2013 Feb 12;41(7):3986–3999. doi: 10.1093/nar/gkt087

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Mechanistics of MEX3A interaction with CDX2 mRNA in AGS cells. (A) Terminal sequence of CDX2 mRNA, with the predicted MRE in the 3′UTR highlighted in bold and underlined. (B) qPCR showing CDX2 and GAPDH mRNAs immunoprecipitated with myc-tag antibody in MEX3A transfected cells (*P = 0.018). The values for CDX2 and GAPDH mRNA levels in the IgG sample were referred to as 1. (C) Schematic representation of pRL constructs. (D) qPCR showing Rluc mRNA immunoprecipitated with myc-tag antibody for pRLCDX2 transfection (****P < 0.0001). Values for Rluc mRNA expression in IgG samples were referred to as 1. (E) Luciferase activity assay for the different pRL constructs (**P = 0.006 for pRLControl/pRLCDX2 and *P = 0.036 for pRLCDX2/pRLΔCDX2). The values obtained for luciferase expression in the pRLControl co-transfected cells were referred to as 1.