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. 2013 Feb 12;41(7):e84. doi: 10.1093/nar/gkt091

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Differential Tet1/2/3 gene expression and global 5hmC levels in ESCs and NPCs. (a) RT-qPCR analysis of Tet1, Tet2 and Tet3 mRNA levels in ESCs and NPCs (mean values ± SD, n = 3). (b) Immunofluorescence analysis of 5hmC levels in ESCs and NPCs. Bar: 50 μm. (c) Dot blot analysis of the global 5hmC levels in the gDNA of ESCs and ESC-derived NPCs. One hundred fifty nanogram gDNA for each dot. (d) The relative 5hmC signal of dot blot and comparative hMeDIP-seq in ESCs and NPCs. *The ratio of hMeDIP/Input reads numbers in ESCs was set as 100%. (e) Distribution of 5hmC in TSS ± 5 kb and gene body regions in ESCs. (f) Distribution of 5hmC in TSS ± 5 kb and gene body regions in NPCs.