Figure 2.

Streptomyces coelicolor antibiotic production in the absence of sIHF. (A) Plasmid-containing wild type (M600) and ΔsIHF in-frame deletion strains, as well as the complemented ΔsIHF-mutant strain, were grown on MS (left) and R2YE (right) agar media for 5 and 4 days, respectively. Images were taken from the underside of each plate to effectively display antibiotic production. (B) Actinorhodin (top) and undecylprodigiosin (bottom) production by wild-type and ΔsIHF-mutant strains in complex liquid YEME-TSB medium over a 6-day time course. (C) CDA production assay by plasmid-containing wild-type and ΔsIHF-mutant strains, along with the complemented ΔsIHF-mutant strain.