Table 1.

Bacterial strains and plasmids

Streptomyces strains	Genotype	Reference
M600	SCP1− SCP2−	Chakraburtty and Bibb (25)
E310	M600 ΔSCO1480(sIHF)::[aac(3)IV)-oriT]	This work
E310a	M600 ΔSCO1480(sIHF)::FRT	This work
Escherichia coli strains	Use	Reference
DH5α	Plasmid construction and general subcloning	Invitrogen
ET12567/pUZ8002	Generation of methylation-free plasmid DNA	Paget et al. (26)
BW25113	Construction of cosmid-based knockouts	Gust et al. (27)
BL21 DE3 pLysS Rosetta	Protein overexpression	Novagen
BT340	DH5α carrying a ‘FLP recombination’ plasmid	Datsenko and Wanner (28)
Plasmids	Description and use	Reference
pIJ2925	General cloning vector	Janssen and Bibb (29)
pIJ773	Plasmid carrying the apramycin knockout cassette	Gust et al. (27)
pIJ790	Temperature sensitive plasmid carrying λ-RED genes	Gust et al. (27)
pIJ8660	Integrating plasmid vector carrying eGFP	Sun et al. (30)
pIJ82	Hygromycin-resistant integrating plasmid vector	Gift from H. Kieser
pET15b	6×His-tag protein fusion overexpression vector	Novagen
pMC153	pIJ8660 + sIHF; sIHF-eGFP localization	This work
pMC154	pIJ82 + sIHF; ΔsIHF complementation plasmid	This work
pMC155	pET15b + sIHF; overexpression of sIHF	This work
pMC141	pET15b + srtA; overexpression of SrtA	Duong et al. (31)
pET11a	Protein overexpression vector	Novagen
pAG8744	pET11a + topA; overexpression of TopA	This work
St9C5	Cosmid for sIHF knockout	Redenbach et al. (32)