Table 1.
The oligonucleotides used in this work
| Abbreviation | Sequence (5′→3′) | Extinction coefficient [L·mol−1·cm−1] | Structure |
|---|---|---|---|
| Hum24a | (TTAGGG)4 | 244 600 | G-quadruplex (monomeric) |
| C-MYCb | TGAGGGTGGGGAGGGTGGGGAA | 229 900 | G-quadruplex (monomeric) |
| KRASc | AGGGCGGTGTGGGAAGAGGGAAGAGGGGGAGG | 341 000 | G-quadruplex (monomeric) |
| Hum48 | (TTAGGG)8 | 489 000 | G-quadruplex (multimeric) |
| Hum54 | (TTAGGG)9 | 550 100 | G-quadruplex (multimeric) |
| Hum59 | TAGGG(TTAGGG)9 | 603 100 | G-quadruplex (multimeric) |
| AT | (AT)6 | 133 300 | Duplex |
| GC | (GC)6 | 101 100 | Duplex |
| LD | GCGCAATTGCGC | 108 700 | Duplex |
| T*AT | 5′-TTTTTTTTTTTTTTTTTT | 146 400 | Triplex |
| 5′-AAAAAAAAAAAAAAAAAA | 219 400 | ||
| 3′-TTTTTTTTTTTTTTTTTT | 146 400 | ||
| Hum24-cd | (CCCTAA)4 | 220 400 | Single-stranded |
| Hum24-Mute | (TTAGAG)4 | 257 000 | Single-stranded |
| C-MYC-cf | TTCCCCACCCTCCCCACCCTCA | 179 900 | Single-stranded |
| ssDNA1 | GAGCTCTCGAAAGAGCTCCGATTA | 235 800 | Single-stranded |
| ssDNA2 | TAGAGCACACCTGTCCGTG | 189 400 | Single-stranded |
aHum24 is a 24-nucleotide G-rich oligonucleotide with the repeated subunit of human telomere.
bC-MYC is a 22-nucleotide G-rich sequence, which is a regulator gene that codes for a transcription factor.
cKRAS is a 32-nucleotide G-rich sequence, which is located in the promoter of the human KRAS gene.
dHum24-c is a C-rich oligonucleotide with a complementary sequence to Hum24.
eHum24-Mut is a variant of Hum24, it cannot fold into G-quadruplex structure.
fC-MYC-c is a C-rich oligonucleotide with a complementary sequence to C-MYC.