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. 2013 Mar 5;41(7):4185–4197. doi: 10.1093/nar/gkt129

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — AROS is specifically required for 40S subunit rRNA processing. (A) Representation of ribosomal RNA maturation including pre-rRNA and rRNA nomenclature. Asterisk indicates the 21S pre-rRNA where processing is stalled by depletion of RPS19. (B) Immunofluorescent detection of AROS (green) and fibrillarin (red) localization in representative MCF7 cells with and without silencing of AROS. Bar is 10 µm. (C) Northern blotting for pre-rRNAs and18S and 28S mature rRNAs. Total RNA from HCT116 cells isolated following siRNA transfection were separated by electrophoresis and blotted using ³²P labelled probes for each pre- or mature rRNA. The negative siRNA targets a splice form of SIRT1, SIRT1Δ8, and illustrates no effect on rRNA processing. (D) Pixel density from whole lanes in (C) calculated using Image J software (National Institutes of Health).