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. 2013 Mar 5;41(7):4185–4197. doi: 10.1093/nar/gkt129

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — AROS is widely expressed in tissues and distributed within cells. (A) Sequence alignment [Clustal Omega (21)] of AROS from 10 species with blue nuclear export [NetNES—(22)] and red nuclear import [NLStradamus—(23)] sequences highlighted. Homo sapiens = human; Pan troglodytes = chimpanzee; Bos Taurus = cow; Canis familiaris = dog; Mus musculus = mouse (all mammal); Gallus gallus = chicken (bird); Xenopus tropicalis = western clawed frog (amphibian); Acyrthosiphon pisum = pea aphid (insect); Anolis carolinensis = Carolina anole (lizard); Danio rerio = zebrafish (fish). (B) Amplification of AROS and RPS19 mRNAs following reverse transcription from a panel of human tissues samples (AMS Biotechnology) were visualized using ethidium bromide staining of agarose TAE gels. (C) Western blotting following subcellular fractionation of HCT116 cells against indicated proteins. Lactate dehydrogenase (LDH) is used as a cytoplasmic control and Lamin AC and Histone H3 as nuclear markers.