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. 2013 Apr 16;11(4):e1001539. doi: 10.1371/journal.pbio.1001539

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© 2013 Milde et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Co-migration was analysed using kymographs (time-distance graphs) obtained from live-cell imaging of neurites from primary culture SCG neurons. Representative kymographs from neurites co-labelled with Nmnat2-EGFP and (A) mito-tagRFP, (B) lamp1-RFP, or Nmnat2-mCherry and (C) LmanI-EGFP, (D) Golga2-EGFP, (E) Syntaxin6-EGFP, (F) TGN38-EGFP, (G) GFP-Bassoon, (H) GAP43-EGFP, (I) SNAP25b-EGFP, (J) Synaptophysin-EGFP, and (K) SynaptotagminI-EGFP. (L) Quantification of co-migration. The quantification shown for each construct represents the percentage of moving Nmnat2-labelled vesicles that were also labelled by the relevant marker. Error bars indicate SEM.