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. 2013 Apr 16;8(4):e61845. doi: 10.1371/journal.pone.0061845

Figure 10. The ability to ectopically induce gem and zic2 is lost in the AB4 mutant.

Figure 10

(A) Ventral ectopic expression of gem and zic2 after injection of each FoxD4L1-AB mutant mRNAs into an epidermal precursor blastomere. Clones are indicated by βGal-positive pink dots. In AB1 and AB2 clones, most cells exhibit a high level of expression (dark blue stain), compared to neighboring cells showing endogenous expression levels (e). Cells in the AB4 clones do not express the genes at levels above endogenous (e). gem-AB1, zic2-AB1, and zic2-AB2 are ventral views with animal cap to the bottom; gem-AB2, gem-AB4, zic2-AB4 are animal cap views. (B) The percentage of embryos in which the FoxD4L1-AB mutants induced gem or zic2 expression in the ventral ectoderm. Labeling is as in 9B.