Figure 3. EGFP-Av-LC3-labeled autophagosomes fuse with endosomes/lysosomes. (A) CEFs were transduced with a recombinant adenovirus expressing avian EGFP-LC3A, -LC3B or -LC3C. After 24 h, cells were incubated in Hank’s balanced salt solution for 2 h to induce autophagy. Endosomes/lysosomes were labeled using anti-LAMP1 (red). Regions of colocalization (boxed) are enlarged below each image. Nuclei (blue) were stained with DAPI. Scale bars: 10 μm. (B) The percent EGFP signal colocalized with LAMP1 was quantified from (A) using Imaris coloc module. Mean plus standard deviation is shown from three independent experiments.