Figure 4. All LC3 paralogs partially colocalize upon induction of autophagic signaling. (A) CK cells were transduced with recombinant adenoviruses encoding both EGFP- and FLAG-mCherry-tagged LC3 paralogs. After 24 h cells were starved for 2 h in HBSS to induce autophagic signaling. Nuclei (blue) were stained with DAPI. Scale bars: 10 µm. (B) The percent EGFP signal colocalized with mCherry signal was quantified from (A) using Imaris coloc module. Open bars, FLAG-mCherry-LC3A; hashed bars, FLAG-mCherry-LC3B; closed bars, FLAG-mCherry-LC3C. Mean plus standard deviation is shown from three independent experiments.