Figure 7. IBV replication is not affected by induction or inhibition of autophagy. CK cells were pretreated for 1 h in full medium (FM), Hank’s balanced salt solution (HBSS), HBSS containing 10 nM wortmannin (HBSS+WM), FM containing 200 nM rapamycin (Rapamycin) or FM containing 10 nM wortmannin (FM+WM). Cells were then infected at (A and B) MOI 10 or (C) MOI 0.1 with IBV, Beau-R strain. At (A) 6 hpi, (B) 12 hpi or (C) 24 hpi, cell supernatant was harvested and titer of released progeny virus was assayed by plaque assay using CK cells. Bars show mean plus standard deviation for three independent experiments. *p = 0.05 in a Student’s t-test; ns, not significant in a Student’s t-test. (D) CK cells were washed and incubated for 1 h in FM, HBSS, HBSS+WM, Rapamycin or FM+WM. Cells were lysed and lysates separated on 10% NuPAGE in MOPS buffer. Proteins were detected by western blot with anti-LC3 or anti-actin.