Figure 4. B cell ATG5 expression is required for appropriate antibody responses during intestinal infection and intestinal inflammation. (A) Age- and weight-matched mice were inoculated with 200 third-stage H. polygyrus larvae. Parasite-specific IgE and IgG1 were quantified by ELISA 1, 2 and 3 weeks post-infection. *p < 0.01; n = 5 per group. (B) Fecal pellets were harvested from age-matched littermates, weighed, and homogenized in PBS at volumes commensurate with the pellet weight. Supernatants were subjected to IgA-specific ELISA for analysis. *p < 0.01; n = 10 per group. Fecal IgA data points shown here correlate to the mice displayed in Figure 2A depicting total serum Ig. (C) Age- and weight-matched mice were given 3% DSS ad libitum in drinking water for 7 d, followed by 5 d of regular drinking water. At day 12, B lymphocytes were isolated from MLN, PP and LP for IgA-specific ELISpot assay analysis. IgA ASCs were enumerated after 24 h and the numbers shown are relative to the number of IgA ASCs present in each tissue from WT mice that received no DSS. *p < 0.001 compared with WT without DSS; n = 7 per group. (D) Body weights plotted from DSS-treated mice in (C). *p < 0.01. Statistics were determined using Student’s t-test.